Journal: Cellular and molecular bioengineering

Article Title: Neurogenic potential of engineered mesenchymal stem cells overexpressing VEGF

doi: 10.1007/s12195-015-0425-4

Figure Lengend Snippet: Endogenous VEGF protein expression was upregulated 4 days post-sciatic nerve transection. Squares, sham surgery. Circles, nerve transection. Data are mean ± SEM, n=4, *p<0.01.

Article Snippet: As a positive control, DRGs were cultured in basal media supplemented with 5 or 100 ng/mL human recombinant VEGF (R&D systems).

Techniques: Expressing

Journal: Cellular and molecular bioengineering

Article Title: Neurogenic potential of engineered mesenchymal stem cells overexpressing VEGF

doi: 10.1007/s12195-015-0425-4

Figure Lengend Snippet: Co-culture of DRG explants, SCs and HAECs with control or VEGF-MSCs increased neurite outgrowth and cell proliferation. (A) Neurite length was increased in DRG explants in co-cultures with control and VEGF-MSCs (*p<0.01 versus basal media). (B) SC proliferation was increased in co-cultures with control and VEGF-MSCs (*p<0.01 versus basal media). (C) HAEC proliferation was increased in co-cultures with control and VEGF-MSCs compared to basal media, and co-cultures with VEGF-MSCs increased proliferation compared to control MSCs (*p<0.01, n≥6). (D–F) Supplementation of cultures with human recombinant VEGF increased cell proliferation in (F) HAEC cultures (*p<0.01) but not (D) DRG explants or (E) SC cultures (n≥7). Data are normalized to their respective basal media controls. Data are mean ± SEM.

Article Snippet: As a positive control, DRGs were cultured in basal media supplemented with 5 or 100 ng/mL human recombinant VEGF (R&D systems).

Techniques: Co-Culture Assay, Control, Recombinant

Journal: Cellular and molecular bioengineering

Article Title: Neurogenic potential of engineered mesenchymal stem cells overexpressing VEGF

doi: 10.1007/s12195-015-0425-4

Figure Lengend Snippet: Addition of the VEGF receptor inhibitor, SU5416, reduced proliferation of HAECs but did not effect neurite extension or SC proliferation. (A) Neurite length of DRG explant cultures. (B) Quantification of SC proliferation. (C) Quantification of HAEC proliferation. Filled columns represent data in the absence of SU5416; open columns represent data in the presence of 0.5 μM SU5416. Data are normalized to their respective basal media controls. Data are mean ± SEM, n≥6, *p<0.01.

Article Snippet: As a positive control, DRGs were cultured in basal media supplemented with 5 or 100 ng/mL human recombinant VEGF (R&D systems).

Techniques:

Journal: Cellular and molecular bioengineering

Article Title: Neurogenic potential of engineered mesenchymal stem cells overexpressing VEGF

doi: 10.1007/s12195-015-0425-4

Figure Lengend Snippet: VEGF-MSCs maintained their overexpression of growth factor and proliferation rate in 3D fibrin gels. (A) VEGF-MSCs maintain VEGF overexpression on tissue culture plastic (TCP) and in fibrin gels. Filled columns: control MSCs; open columns: VEGF-MSCs (n=6, *p<0.01 versus control MSCs). (B) Proliferation of control and VEGF-MSCs was not different when cultured on TCP or in fibrin gels. Filled columns, control MSCs. Open columns, VEGF-MSCs. (n=4). (C) Conduits filled with VEGF-MSCs embedded within fibrin gels secrete detectable levels of VEGF for up to 21 days (n=4). Data are mean ± SEM. Fluorescent images of conduits filled with green fluorescent protein (GFP)-positive VEGF-MSCs embedded within fibrin gels at Day (D) 0, (E) 6, (F) 12, and (G) 21. Scale bar=100 μm.

Article Snippet: As a positive control, DRGs were cultured in basal media supplemented with 5 or 100 ng/mL human recombinant VEGF (R&D systems).

Techniques: Over Expression, Control, Cell Culture

Journal: Cellular and molecular bioengineering

Article Title: Neurogenic potential of engineered mesenchymal stem cells overexpressing VEGF

doi: 10.1007/s12195-015-0425-4

Figure Lengend Snippet: GFP-positive MSCs remained localized at the site of implantation at week 2. Confocal images of transected sciatic treated with a PLLA conduit containing (A) fibrin alone, (B) a fibrin matrix with control MSCs, or (C) a fibrin matrix with VEGF-MSCs. Conduits are oriented with the proximal end on the left and the distal end on the right. Green, GFP for endogenous green fluorescent protein positive-MSCs. Blue, DAPI for cell nuclei. Scale bar = 500 μm.

Article Snippet: As a positive control, DRGs were cultured in basal media supplemented with 5 or 100 ng/mL human recombinant VEGF (R&D systems).

Techniques: Control

Journal: Cellular and molecular bioengineering

Article Title: Neurogenic potential of engineered mesenchymal stem cells overexpressing VEGF

doi: 10.1007/s12195-015-0425-4

Figure Lengend Snippet: Conduits filled with VEGF-MSCs embedded within fibrin gels maintain VEGF overexpression 2 weeks post-transplantation. Filled columns: control MSCs; open columns: VEGF-MSCs. Data are mean ± SEM, n≥3, *p<0.01.

Article Snippet: As a positive control, DRGs were cultured in basal media supplemented with 5 or 100 ng/mL human recombinant VEGF (R&D systems).

Techniques: Over Expression, Transplantation Assay, Control